Prevention of nosocomial COVID-19 infections in otorhinolaryngology-head and neck surgery.

Coronavirus disease 2019 (COVID-19) has rapidly evolved into a pandemic, causing a global public health crisis. Many frontline healthcare workers providing ear, nose and throat services have been reported to contract COVID-19 at work. Early during the COVID-19 outbreak, several medical professionals in Otolaryngology-Head and Neck Surgery were infected in Wuhan, China. A series of measures were then taken immediately, which successfully halted the spread of the disease. Here we would like to share the lessons we have learned, and our experience to protect our health care workers during the COVID-19 pandemic.

Structural Basis of a Human Neutralizing Antibody Specific to the SARS-CoV-2 Spike Protein Receptor-Binding Domain.

The emerging new lineages of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) have marked a new phase of coronavirus disease 2019 (COVID-19). Understanding the recognition mechanisms of potent neutralizing monoclonal antibodies (NAbs) against the spike protein is pivotal for developing new vaccines and antibody drugs. Here, we isolated several monoclonal antibodies (MAbs) against the SARS-CoV-2 spike protein receptor-binding domain (S-RBD) from the B cell receptor repertoires of a SARS-CoV-2 convalescent. Among these MAbs, the antibody nCoV617 demonstrates the most potent neutralizing activity against authentic SARS-CoV-2 infection, as well as prophylactic and therapeutic efficacies against the human angiotensin-converting enzyme 2 (ACE2) transgenic mouse model in vivo. The crystal structure of S-RBD in complex with nCoV617 reveals that nCoV617 mainly binds to the back of the "ridge" of RBD and shares limited binding residues with ACE2. Under the background of the S-trimer model, it potentially binds to both "up" and "down" conformations of S-RBD. In vitro mutagenesis assays show that mutant residues found in the emerging new lineage B.1.1.7 of SARS-CoV-2 do not affect nCoV617 binding to the S-RBD. These results provide a new human-sourced neutralizing antibody against the S-RBD and assist vaccine development. IMPORTANCE COVID-19 is a respiratory disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The COVID-19 pandemic has posed a serious threat to global health and the economy, so it is necessary to find safe and effective antibody drugs and treatments. The receptor-binding domain (RBD) in the SARS-CoV-2 spike protein is responsible for binding to the angiotensin-converting enzyme 2 (ACE2) receptor. It contains a variety of dominant neutralizing epitopes and is an important antigen for the development of new coronavirus antibodies. The significance of our research lies in the determination of new epitopes, the discovery of antibodies against RBD, and the evaluation of the antibodies' neutralizing effect. The identified antibodies here may be drug candidates for the development of clinical interventions for SARS-CoV-2.

COVID-19 and energy: Influence mechanisms and research methodologies.

Considering the important role of energy in modern society, it is imperative to study the current situation and future development of energy under the influence of COVID-19. This paper identifies the current research hotspots, proposes future research directions accordingly, and summarizes the methodologies via a bibliometric analysis. Five research hotspots include COVID-19 and the changes of energy consumption, COVID-19 and the fluctuation of the energy market, COVID-19 and the development of renewable energy, COVID-19 and climate impacts caused by energy consumption, and COVID-19 and the energy policy. According to the influence mechanism of COVID-19 on each hotspot, the pandemic has exerted short-term influencs on energy consumption, energy price, and air pollution. Meanwhile, the pandemic could have a far-reaching impact on the renewable energy sector, climate, and energy policy. In addition, the main methodologies are reviewed, revealing that regression analysis and scenario analysis are commonly used as the quantitative and qualitative methods, respectively. Moreover, given the nonlinear relations between the pandemic and energy, an artificial neural networks model is used to enhance the prediction efficiency of energy demand and price. Finally, policy implications for obtaining clean, low-carbon, safe, and efficient energy in the context of COVID-19 are proposed.

A SARS-CoV-2 antibody curbs viral nucleocapsid protein-induced complement hyperactivation.

Although human antibodies elicited by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleocapsid (N) protein are profoundly boosted upon infection, little is known about the function of N-reactive antibodies. Herein, we isolate and profile a panel of 32 N protein-specific monoclonal antibodies (mAbs) from a quick recovery coronavirus disease-19 (COVID-19) convalescent patient who has dominant antibody responses to the SARS-CoV-2 N protein rather than to the SARS-CoV-2 spike (S) protein. The complex structure of the N protein RNA binding domain with the highest binding affinity mAb (nCoV396) reveals changes in the epitopes and antigen's allosteric regulation. Functionally, a virus-free complement hyperactivation analysis demonstrates that nCoV396 specifically compromises the N protein-induced complement hyperactivation, which is a risk factor for the morbidity and mortality of COVID-19 patients, thus laying the foundation for the identification of functional anti-N protein mAbs.

The role of weather conditions in COVID-19 transmission: A study of a global panel of 1236 regions.

It is believed that weather conditions such as temperature and humidity have effects on COVID-19 transmission. However, these effects are not clear due to the limited observations and difficulties in separating impact of social distancing. COVID-19 data and social-economic features of 1236 regions in the world (1112 regions at the provincial level and 124 countries with the small land area) were collected. Large-scale satellite data was combined with these data with a regression analysis model to explore the effects of temperature and relative humidity on COVID-19 spreading, as well as the possible transmission risk by seasonal cycles. The result shows that temperature and relative humidity are negatively correlated with COVID-19 transmission throughout the world. Government intervention (e.g. lockdown policies) and lower population movement contributed to decrease the new daily case ratio. Weather conditions are not the decisive factor in COVID-19 transmission, in that government intervention as well as public awareness, could contribute to the mitigation of the spreading of the virus. So, it deserves a dynamic government policy to mitigate COVID-19 transmission in winter.

Current Status and Practical Considerations in the Development of Convalescent Plasma as a Treatment for COVID-19

Abstract The ongoing COVID-19 pandemic caused by rapid spreading of the SARS-CoV-2 virus since December 2019 is inflicting tremendous harm in human health and causing severe economic damages around the world. Expeditious identification and development of intervention therapies is of great importance before safe and effective vaccines against SARS-CoV-2 become widely available. Convalescent plasma therapy has emerged as a promising candidate that could be safe and effective against COVID-19 within a short period of time, due to positive experiences thus far with regard to both safety and efficacy from past infectious disease outbreaks. This review provides a narrative summary of existing clinical data for the efficacy and safety of convalescent plasma in infectious diseases, the current status of its development and use as a COVID-19 treatment, and some practical considerations in the design of clinical trials. The experiences learned about convalescent plasma as a therapy from the past outbreaks and the ongoing COVID-19 pandemic will be invaluable in combating future outbreaks of infectious diseases.